A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
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منابع مشابه
Isolation of genomic DNA suitable for community analysis from mature trees adapted to arid environment.
Isolation of intact and pure genomic DNA (gDNA) is essential for many molecular biology applications. It is difficult to isolate pure DNA from mature trees of hot and dry desert regions because of the accumulation of high level of polysaccharides, phenolic compounds, tannins etc. We hereby report the standardized protocol for the isolation and purification of gDNA from seven ecologically and me...
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Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissuesof Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturitywere analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA qua...
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Background & Aim:Combretaceae family is known for efficacy in the management of sickle cell disorder (SCD). Based on the earlier reports of anti-sickling property of Terminalia genus the study investigates in vitro antisickling properties of Terminalia arjuna and Terminalia bellirica. Experimental: Anti-sickling ...
متن کاملAn efficient and simple CTAB based method for total genomic DNA isolation from low amounts of aquatic plants leaves with a high level of secondary metabolites
An efficient DNA isolation protocol specifically modified to get pure quality DNA required for molecular studieshas been reported in this paper. Some aquatic plants (Potamogeton spp., Ceratophyllum demersum and Myriophyllum spicatum) were used for the study. The protocol developed will be useful in getting high and pure DNA. Instead of using the available DNA extraction kits, this protocol can ...
متن کاملAn in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica
Background & Aim:Combretaceae family is known for efficacy in the management of sickle cell disorder (SCD). Based on the earlier reports of anti-sickling property of Terminalia genus the study investigates in vitro antisickling properties of Terminalia arjuna and Terminalia bellirica. Experimental: Anti-sickling ...
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تاریخ انتشار 2006